Foxp3 Detection

GIVI
The Gladstone Institutes
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience™ from Invitrogen Antibodies

Product Name:

APC Anti-mouse Foxp3

Catalog Number:

17-5773-80

I was interested in detecting the induction of naive CD4 T-lymphocytes in vitro to regulatory T-cells and used this marker to look at its differentiation by flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Isolated mouse CD4 T-cells inducted in vitro

Primary Incubation

3 hour fix perm buffer

Blocking Agent

FC block

Secondary Incubation

1:200 dilution, 30 min at 4 C

Tertiary Incubation

None

Detection

Flow Cytometer

Results Summary

Very clear results that presented a distinct population of Foxp3+ cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very reliable, one of the best

The Bad

None

The Bottom Line

Highly recommended antibody for looking at populations of regulatory T-cells

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