LGP2 Antibody That Works for Mouse and Human Blots and Immunoprecipitation

June 22, 2017

Immunology
University of Washington
Senior Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Proteintech Group

Product Name:

LGP2 Rabbit Polyclonal Antibody

Catalog Number:

11355-1-AP

I study LGP2 in innate immunity. For this experiment, I was testing the ability of various antibodies to pull down LGP2 in an overexpression system. This product was one of several tested.

Experimental Design and Results Summary

Applications

Immunoprecipitation

Sample

Flag-tagged LGP2 overexpression from HEK cells

Primary Incubation

10 uL antibody with 50 uL magnetic beads, then overnight incubation with cell lysis at 4C rotating end over end

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

anti-Flag (M2 clone) 1:1000 1hr Room Temp followed by Donkey anti-mouse AlexaFluor790 secondary 1:10,000 1 hr Room temp. Detected using LiCor Odyssey.

Results Summary

LGP2 bands from IP are shown by Western blot. Several suppliers of antibodies for the IP were compared to determine the most efficient pulldown. Proteintech Group's antibody had the best pulldown compared to the other suppliers.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Clean antibody, no crossreactivity, excellent for pulldowns and western blots

The Bad

May need to increase antibody concentration in different cell types

The Bottom Line

Solid, reliable antibody.

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