Phosphorylation Of STAT3 In Mouse B Cells

May 26, 2017

Immunology
Northwestern University
Grad Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biolegend

Product Name:

PE anti-STAT3 Phospho

Catalog Number:

651004

The aim of the research was to determine phosphorylation of STAT3 in B cells after different stimuli.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenic B cells

Primary Incubation

5ul (one test), 4 degrees, 1 hour

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

None

Results Summary

Clearly shows phosphoSTAT3 staining after stimulating cells with IL-21. No optimization, followed manufacturer's recommendation.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use, clearly marks positive cells

The Bad

None

The Bottom Line

Great antibody for measuring phosphoSTAT3 by flow cytometry.

Share your experience with other scientists. Write a Review! »

Join the discussion