Mic qPCR Cycler

April 24, 2017

DNA Diagnostic
R&D
Research Scientist

Overall

Performance

Ease-of-Optimization

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Company:

Bio Molecular Systems

Product Name:

Mic qPCR Cycler

Catalog Number:

MIC-4

To determine if the DNA Diagnostic qPCR kits, the HemaVision Q-series and Salmonella Velox, perform well on the MIC qPCR by Bio Molecular Systems, a comparison study was executed February-March 2017 at DNA Diagnostic A/S Denmark.
The comparison study compared samples on an ABI 7500 Fast instrument (reference) and a MIC qPCR instrument. The comparison concluded that the HemaVision Q-series and Salmonella Velox qPCR mixes performs very well on the MIC qPCR instrument using MIC qPCR tubes.
We found the instrument easy to use and the software intuitive.

Experimental Design and Results Summary

Application

Detection of salmonella spp. (Salmonella Velox) and detection of translocations associated with leukemia (HemaVision 7Q)

Starting Material

DNA extraction of Salmonella Velox Broth with raw meat and three levels of salmonella contamination (Salmonella Velox) and in vitro transcribed RNA (HemaVision)

Protocol Overview

The comparison study compared samples on an ABI 7500 Fast instrument (reference) and a MIC qPCR instrument

Tips

Note that the MIC uses 'special' tubes which are in strips of 4.

Results Summary

Example: Salmonella Velox qPCR on Salmonella Velox DNA extraction of Salmonella Velox Broth with raw meat and three levels of salmonella contamination. Figures display the amplification curves (directly from the supplier software) of the ROX channel (salmonella target), showing the three salmonella levels in two technical replicates.The comparison concluded that the HemaVision Q-series and Salmonella Velox qPCR mixes performs very well on the MIC qPCR instrument using MIC qPCR tubes.

Features Summary

Easy to use, small, great performance, 4 channels

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Price, performance, ease of use, and much more.

The Bad

The tube format present some limitations in general pipetting

The Bottom Line

The MIC qPCR system is suitable for customers using the DNA Diagnostic HemaVision Q-series and Salmonella Velox kits.Overall: It's a great little instrument.

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