PE/Cy7 Anti-Mouse IL-12/IL-23 P40 Antibody

April 13, 2017

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE/Cy7 anti-mouse IL-12/IL-23 p40 (monomer, dimer, heterodimer) Antibody

Catalog Number:

505209

I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-mycobacterial ESAT6 binding peptides.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Cells

Primary Incubation

1 hour on ice with surface stain antibodies

Blocking Agent

Blocking with 2% BSA and Fc block (1:100)

Secondary Incubation

1:50 dilution on ice with intracellular staining antibodies

Tertiary Incubation

NA

Detection

FACS

Results Summary

Significant intracellular IL-12 p40 levels were detected.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Good flow cytometry antibody for IL-12 intracellular staining

The Bad

None

The Bottom Line

Good antibody for IL-12 intracellular staining T cells.

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