Genscript
THETM DYKDDDDK Tag Antibody, mAb, Mouse
A00187-200
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THETM DYKDDDDK Tag Antibody was used to detect an DYKDDDDK tagged construct in Hela cells. The staining was robust and specific. The antibody was comparable to Sigma's M2 antibody but it is a different isotope.
Immunofluorescence
HeLa cells fixed with 3.7% paraformadehyde
1 hour at room temperature in blocking agent at 1:300
PBS +1% normal goat serum and 0.1% Triton X-100
Goat anti-mouse conjugated to Alexa 488
DAPI
Confocal microscopy
THETM DYKDDDDK Tag Antibody showed robust staining to an overexpressed epitope tagged construct
N/A
Good alternative to M2 and a different mouse isotype
None
A good alternative to M2 (IgG1) if you want to use a different isotype (IgG2b).