Review Of Thy1.2 (CD90.2) Brilliant Violet 510

April 10, 2017

Research
Bloodworks NW
Postdoctoral research fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 510™ anti-mouse CD90.2 Antibody

Catalog Number:

105335

We selected this antibody to detect T cells expressing the Thy1.2 congenic markers in our mixed bone marrow chimeras. Given that the Thy markers are known to be expressed at a high level on T cells, we decided to pair the Thy1.2 marker with a medium-bright fluorochrome such as BV 510.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Splenocytes

Primary Incubation

30 mins @ +4C

Blocking Agent

anti-CD16/32

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

Shown here is a titration performed on splenocytes from 1:100 to 1:1600. The antibody gives some background staining at 1:100-1:200 but improves from 1:400 to 1:1600. Based on the histogram plots and calculations for signal to noise ratios, we decided to use 1:400 for all our subsequent experiments.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very bright, good separation between positive and negative populations.

The Bad

Negative population is pretty spread out. Significant spillover into BV605/SB600 channel.

The Bottom Line

Good antibody. Use caution when designing staining panel. Do take into account the significant spillover into the BV605/SB600 channel.

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