The Good
Directly labelled with a fluorophore. Manufacturer provides recommended dilutions for testing, thus minimizing optimization time. Works with several species, thus allowing the same reagents to be used if you are performing both animal and human studies/experiments simultaneously.
The Bad
This antibody recognizes an Intracellular epitope, thus cell fixation and permeabilization are required which results in longer assay times and may not be compatible in multi-staining experiments involving several antibodies.
The Bottom Line
This antibody stained CD3 fairly well on human PBMC for flow cytometry. Separation between CD3- and CD3+ cells was just OK. There are other products available that result in better separation between the two populations. The benefit of this product is that it is reported by the manufacturer to work with several species. Therefore, if your lab simultaneously utilizes several species for your studies, this antibody might be appealing.