Abcam
Anti-Amyloid Precursor Protein antibody [Y188]
ab32136
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Our lab performs the cuprizone model of toxicity to assess neuroinflammation, demyelination/remyelination, and axonal damage in various mouse lines. To assess axonal damage, we often use APP immunostaining and count axonal swellings by IHC.
Immunohistochemistry
Frozen coronal sections of mouse brain
Anti-Amyloid Precursor Protein antibody [Y188] @ 1:100
5% milk 5% goat serum
Goat anti-rabbit Alex Fluor-633 at 1:1000
DAPI
Light microscopy
Axonal spheroids are clearly visible in sections containing damaged axons.
N/A
Stains axonal spheroids very well when used at an optimal dilution
None
Great antibody for staining axonal spheroids in damaged axons.