ThermoFisher
Isolectin GS-IB4 From Griffonia simplicifolia, Alexa Fluor® 594 Conjugate
I21413
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Our lab studies vessel growth in the mouse retina. We like to stain for other cells as well and need a good fluorescent vessel marker.
Immunofluorescence
Mouse retina cells
Mouse retina was fixed with 10 formalin over night at 4 degrees. After washing with PBS, retinas were incubated with the antibody in staining buffer (1:200) for 2 hours at room temperature in staining buffer (or over night at 4 degrees Celsius).
Do not stain longer than 2 hours at room temperature to avoid high background
Using this antibody we are able to successfully visualize vessel growth in the mouse retina and it does not cause much spillover in other channels.
N/A
Bright color
None
This is a good antibody to use to visualize vessels in fixed mouse tissue with low background.
Product Name: Isolectin GS-IB4 From Griffonia simplicifolia, Alexa Fluor 594 ConjugateSupplier Page