EMD Millipore
Anti-Neurofilament H Non-Phosphorylated Mouse mAb (SMI-32)
NE1023
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Our lab uses the cuprizone model of toxicity to examine demyelination and remyelination and the EAE model to examine neuroinflammation in murine models. The SMI32 antibody is a marker of axonal damage and can be seen as axonal spheroids in tissue sections.
Immunohistochemistry
Paraffin-embedded mouse lumbar spinal cord sections
Anti-Neurofilament H Non-Phosphorylated Mouse mAb (SMI-32) at 1:5000
5% milk 5% goat serum
VECTASTAIN ABC HRP Kit (Peroxidase, Mouse IgG ) at 1:1000
DAB
Light microscopy
When used at an optimized dilution, axonal damage can be visualized as spheroids.
N/A
Stains axonal spheroids very well when used at an optimal dilution
If too much antibody is used, it can cross react with phosphorylated neurofilament
Great antibody for assessing axonal damage