Great Anti-FAK (Phospho Y397) Antibody

March 20, 2017

Physiology and Biophysics
Albert Einstein College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Phospho-FAK antibody

Catalog Number:

ab39967

We have used this antibody to stain for phospho-FAK in U2OS cells fixed with PFA. We localized focal adhesion proteins relative to this stain.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PFA Fixed U2OS cells

Primary Incubation

1:500 at room temperature for 1.5 hours

Blocking Agent

5% NGS

Secondary Incubation

1:750 rhomadine conjugated anti-rabbit antibody

Tertiary Incubation

None

Detection

Confocal Microscope

Results Summary

Phospho FAK (shown here in red) clearly localized to the focal adhesions. Microtubules (green) grow along the expansive leading edge.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Quality staining at a low concentration.

The Bad

None. Clean.

The Bottom Line

Works well to stain the Phospho-FAK protein after PFA fixation.

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