Nice Antibody To Detect Germinal Center B Cells

Microbiology
Medical College of Wisconsin
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse CD95 (Fas) Antibody

Catalog Number:

152608

We often use this antibody to examine germinal center B cell expansion following viral infection of mice. When used in combination with GL7 and B220 markers, we can reliably distinguish germinal center B cells from other splenic cell populations.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:500 - 25 minutes on ice

Blocking Agent

FcR blocking antibody

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII

Results Summary

Works great. We reliably observe ~3% of B220+ cells staining positive for CD95-PE and GL7-FITC at relevant time points post infection.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Reliable, easy to use

The Bad

PE is not always the best fluorochrome option

The Bottom Line

This antibody has been pretty reliable. Works great with our GL7-FITC and B220-PB antibodies. One of our most commonly used antibodies.

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