Okay Antibody To Stain For Mouse CD4+ T Cells

March 14, 2017

Pediatrics
Stanford University
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 510™ anti-mouse CD4 Antibody

Catalog Number:

100449

Successfully detects CD4+ T cells in thymus, spleen, and lymph node with good signal but comes with some background.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:100 dilution on ice for 20-30 min

Blocking Agent

PBS + 2% FBS + 1 mM EDTA

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry violet laser

Results Summary

Successfully stained for mouse CD4+ T cells in splenocytes with high specificity and has good signal. Served as a single stain control.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good specificity and signal

The Bad

High background, doesn't fare very well when fixing cells

The Bottom Line

A good antibody for detecting mouse CD4+ T cells. However, there is some background and it loses some signal upon fixation.

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