Good Antibody to Stain for Mouse CD4+ T Cells

Pediatrics
Stanford University
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 421™ anti-mouse CD4 Antibody

Catalog Number:

100438

Successfully detects CD4+ T cells in thymus, spleen, and lymph node with high signal. Used as a single stain for compensation when analyzing multiple mouse cell types.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Splenocytes

Primary Incubation

1:100 dilution on ice for 20-30 min

Blocking Agent

PBS + 2% FBS + 1 mM EDTA

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry violet laser

Results Summary

Successfully detects CD4+ T cells in in the spleen with high specificity. Does not lose signal when fixed with 4% paraformaldehyde and permeabilized with 0.5% saponin.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Great signal with low background

The Bad

None

The Bottom Line

A great antibody to stain for mouse CD4+ T cells.

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