TaqMan Reverse Transcription Reagent Kit By Applied Biosystems

March 13, 2017

Albert Einstein College of Medicine
Microbiology & Immunology
Post-doctoral Fellow

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Quality of Results

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Company:

Applied Biosystems

Product Name:

TaqMan Reverse Transcription Reagents

Catalog Number:

N808-0234

Our laboratory uses AB's TaqMan reverse transcription reagents/kit for all of our qRT-PCR needs. The kit provides built-in normalization and has flexible, diverse utility (includes reagents for both random hexamer and oligo(dT) protocols). The kit is easy to use and has consistently provided us with accurate, clean results.

Experimental Design and Results Summary

Application

qRT-PCR, RT PCR

Starting Material

mRNA

Protocol Overview

Thaw all required reagents (add the enzymes last/keep at -20C as long as possible) and prepare the master mix according to the protocol guidelines (volumes) outlined in the manual. Aliquot the master mix to clean PCR tubes, and subsequently add the primers and the appropriate RNA template(s) to each tube. Mix the tubes, centrifuge, and place in the thermalcycler. Incubate the tubes according to the suggestions outlined in the manual (dependent on primer format chosen).

Tips

Handle all items with proper care/caution to avoid RNA degradation.

Results Summary

The quality of the cDNA can be determined following downstream applications (i.e. qRT-PCR). NanoDrop can also be used to assess sample concentration and purity following the reverse transcription reaction.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy to use, quick/time efficient protocol, consistent, specific results.

The Bad

Like all kits, expensive.

The Bottom Line

AB's TaqMan reverse transcription kit is perfect for anyone performing qRT-PCR that wants specific, consistent, and clean results. The reagents are reliable, and the entire protocol is easy to follow and time efficient. The kit also has diverse utility, making it ideal for lab's that use different qRT-PCR or RT-PCR protocols.

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