Excellent Antibody For Detection Of Human BRAF Protein

February 14, 2017

Cardiology
BIDMC
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-B Raf antibody [EP152Y]

Catalog Number:

ab33899

The aim of the study was to detect BRAF protein expression in human iPSC-derived cardiomyocytes by Western Blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human iPSC-derived cardiomyocytes (3 different samples)

Primary Incubation

Dilution 1/2000 overnight at 4 degrees

Blocking Agent

5% BSA

Secondary Incubation

Anti-rabbit HRP antibody diluted 1/5000, 1 hour at RT

Tertiary Incubation

NA

Detection

ECL(Pierce)

Results Summary

This antibody detected BRAF at the correct expected size ( around 85 kDa). Specifically, two bands can be detected which suggests that a fraction of BRAF proteins was modified through a post-translational mechanism such as phosphorylation or glycosylation.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

Very specififc antibody for the detection of human BRAF protein

The Bad

The antibody gives a faint unspecific band around 100 kDa

The Bottom Line

I would definitely recommend this antibody for detection of BRAF in human cells. This antibody was here used at 1/2000 dilution but could likely be used at a even higher dilution.

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