Invitrogen by ThermoFisher Scientific
Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 546
A10040
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The aim of this experiment was to detect the Nkx2-1 protein in mouse lung tissue. The goal was to confirm the expression of Nkx2-1 protein in embryonic mouse lung. This secondary antibody was recommended by colleagues, low background and easy to optimize as well as detect.
Immunohistochemistry
Formalin Fixed Paraffin Embedded Tissue Sections of Mouse Embryonic lung
N/A
Donkey Serum
1:200, 2 hours at room temperature in the dark.
Microrscopy
Cells were positively stained for Nkx2-1 in embryonic mouse lung tissue.
Ease of use
Great shelf life and good for immunohistochemistry applications.