Invitrogen by ThermoFisher Scientific
Donkey anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 488
A21202
Info: View Product View Product Specs
Browse Similar Products: IgG (H L) Immunohistochemistry Antibodies
The aim of this experiment was to detect the expression of Sox9 protein in formalin fixed paraffin embedded mouse embryonic lung tissue. Therefore, specific Sox9 primary antibody was used for the immunostaining process and the Donkey anti-Mouse was used as the secondary detection antibody (Alexa Fluor 488). Positive cells were stained green in the distal region of the lung. This secondary antibody was recommended by colleagues and is routinely used in the lab.
Immunohistochemistry
Formalin Fixed Paraffin Embedded Tissue Sections of Mouse Embryonic lung
N/A
Donkey Serum
1:200, 2 hours at room temperature in the dark.
Microrscopy
Sox9 positive cells were stained green.
Cover the tissue sections with foil during the secondary antibody incubation process.
Ease of use
High background.
Secondary antibody titration needs to be performed per application to determine the right concentration especially as there is high background detected.