Successful MMP-9 measurements

January 06, 2017

University of Louisville
Microbiology & immunology
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Boster

Product Name:

Human MMP-9 PicoKine™ ELISA Kit

Catalog Number:

EK0465

The instructions are clear, concise, and the process is more streamlined than other kits. I highly recommend investing in these kits compared to other, less expensive ones.

Experimental Design and Results Summary

Application

Test the quantity of MMP-9 released from neutrophils after challenge with bacteria

Starting Material

Human Neutrophils

Protocol Overview

1. collect supernatant from bacteria-challenged neutrophils2. Run samples on MMP-9 ELISA kit3. Read results on plate reader

Tips

Frozen supernatants work just as well as fresh ones

Results Summary

I highly recommend the Boster line of ELISA kits because of their cost and their effectiveness. I had one last round of experiments to run for a publication and we regrettably decided to purchase a lower cost kit from another company. I ran the same samples on two different plates from that company and even my controls were all over the place, resulting in a waste of time and money. This is when we purchased the Boster kit, and by only using one plate, I was able to see the real results of my experiment. My negative and positive controls had the necessary jump and my samples followed the expected trend.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Clear concise instructions, pre-prepared reagents, shorter incubation times

The Bad

The colorimetric step is variable so if you wait until the suggested incubation time, your top standard may be unreadable

The Bottom Line

Great product, reasonably priced, gets consistent, real results.

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