Expression of cIAP1 in HEK293 cells

Pathology
University of Tennessee Health Science Centre
Postdoctoral Fellow

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Company:

Cell Signaling Technology

Product Name:

Anti-cIAP1 Antibody

Catalog Number:

7065

cIAP1 is also named BIRC2, cellular inhibitor of apoptosis protein-1. It belongs to the IAP family of proteins and therefore contains at least one BIR (baculoviral IAP repeat) domain. cIAP1 binds with caspases and inhibits apoptosis. The present study aims to check the expression of cIAP1 in HEK293 cells by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK293 cells

Primary Incubation

1:1000

Blocking Agent

No blocking

Secondary Incubation

1:5000

Tertiary Incubation

NA

Detection

ECL

Results Summary

Antibody (CST#7065) detected cIAP1 as single clear band at around 62 kDA in HEK293 cells by western blot.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Identifies clear band at right size

The Bad

Nothing

The Bottom Line

Antibody (CST#7065) produced cIAP1 as clean single band at around 62 kDA.

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