Use Of N-Cadherin (CDH2, ACAM) Antibody From Sigma-Aldrich On Embryonic Tissue

November 18, 2016

Pathology
BWH
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

Monoclonal Anti-N-Cadherin antibody (clone GC4)

Catalog Number:

C3865

This antibody was used to analyze the distribution of N-Cadherin and cell adhesion in embryonic tissues. This monoclonal anti N-Cadherin (clone GC4) antibody appears highly specific to cell membrane and is expressed at variable intensity depending the tissue (neural tube, mesoderm,etc..).

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Chicken embryonic tissue section

Primary Incubation

1:250, overnight, at 4C in Blocking agent, on slide

Blocking Agent

TBS, 1% FBS, 0.1% TritonX100, 30min, at RT

Secondary Incubation

1:500, AlexaFluor-594-conjugated anti-mouse IgG secondary (Molecular Probes), overnight, at 4C in Blocking agent, on shaker

Tertiary Incubation

N/A

Detection

Fluorescent microscope, TexasRed filter for AlexaFluor-594 detection

Results Summary

This antibody works very well on fixed tissue section of embryos. High specificity for the cell membrane, marks mostly mesoderm and neural tube, both epithelia and mesenchymes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong specific signal at cell membrane by immunofluorescence

The Bad

None known

The Bottom Line

Excellent antibody overall. Highly recommended for immunofluorescence study of epithelial-mesenchyme structures on tissue sections. This antibody reacts with N-Cadherin (CDH2) essentially distributed at the membrane and cell-cell contacts.

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