A PCNA antibody for Western blotting

November 02, 2016

Pharmacology
Boston University
Senior Post-doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

PCNA (D3H8P) XP Rabbit Antibody

Catalog Number:

13110S

PCNA is a DNA clamp protein complex that assists in DNA replication. PCNA is expressed in late G1 and reaches its maximal level in S phase, thus can be used as a marker of cells that are actively replicated in S phase.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK293T

Primary Incubation

1:1000 in TBS-T at Room Temperature for 2hr

Blocking Agent

5% milk in TBS-T

Secondary Incubation

Anti-Rabbit-HRP at 1:8000 in TBS-T

Tertiary Incubation

N/A

Detection

Bio-Rad ECL

Results Summary

The antibody specifically detect a single band between markers 37 and 25 kDa. The image was taken in few seconds indicating the strong sensitivity of this antibody.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Great specificity and sensitivity

The Bad

N/A

The Bottom Line

This rabbit monoclonal antibody recognizes PCNA with a great specificity and sensitivity. Only minimal amount of antibody is needed to obtain a nice blot. Overall, this antibody exceeds my expectation.

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