BioLegend
PE anti-human CD137 (4-1BB) Antibody
309803
Info: View Product View Product Specs
Browse Similar Products: CD137 Flow Cytometry Antibodies
We are building multi-color flow panels to characterize activation of pTFH cells in chronic HIV infection. We chose to use CD137-PE to detect activated T cells in response to stimulation and it fit well in our 10-color panel.
Flow Cytometry
Human PBMC
0.1uL CD137-PE in 200uL PBS with 10^6 cells; 15 minutes; room temperature
None.
BD LSRFortessa
0.1uL CD137-PE worked well to detect activated T cells after overnight stimulation with SEB (1ug/mL). It yielded the expected frequency of CD137+ CD4+ T cells after an overnight stimulation (~2-3%). CD137-PE worked well in a panel of 10 conjugates and didn't cause any bleedover (as evidenced by FMO controls).
None
Cost effective (0.1uL works great); plays well with other colors at 0.1uL
CD137-PE works great to measure T cell activation.