Boster
Anti-CD10/Neprilysin Antibody
PB9058
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Browse Similar Products: CD10 Western Blot Antibodies
We selected this product as a polyclonal ab for our Tg Rat colony. We are interested in adding enzymatic catabolism/clearance of amyloid beta isoforms into our body of research. We purchased this product because it was cheap and we are looking at some 'proof-of-concept' and didn't need remarkable specificity. We still have much to investigate.
Western Blot
Rat PFC
Several, to determine optimal protocol (1:25-1:100).
Protein simple proprietary "antibody diluent II'
Anti rat, 1:150
None
Protein Simple, Simple Western size-based assay
This antibody produced several bands, the most prominent of which occurred at about 75kDa (supposedly our protein of interest), a dual band around ~50kDa, and a dimmer, but present band at around 32kDa. There was a large amount of smearing from 230 to the 75kDa mark.
The images represent the ab at 1:25 in increasing protein concentrations. Secondary ab at 1:150
Cheap, prompt shipping
Lacking specificity with the Wes protocol
We still have not tested this using a classic western