Cell Signaling Technology
Phospho-PKD/PKCμ (Ser744/748) Antibody
2054
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Protein Kinase D (PKD) is family of Ser/Thr kinases that regulate multiple biological functions including cell migration, proliferation, apoptosis, transcriptional activation, angiogenesis, and Golgi organization and vesicle trafficking. Like many other kinases, PKD functions are mediated via phosphorylation of different substrates. The goal of this project was to demonstrate if rhotekin was a bona fide substrate of PKD and whether this phosphorylation is important for regulating RhoA. In this experiment, phosphorylation of both PKD and rhotekin was tested after activating PKD with PMA.
Western Blot
PANC-1 cells
1:1000 dilution in blocking buffer; 16 hr at 4 deg
5% BSA in 1xTBS-T(0.1%)
Goat-anti Rabbit-IgG-HRP conjugated
None
ECL pico
After PMA addition for 10 min, PKD was found to be phosphorylated at position Ser744/748 an indication that the kinase is active.
Works as stated
Very good antibody to test PKD activity