His tag antibody for Western Blotting

Protein Kinase D is family of Ser/Thr kinases that regulate multiple biological functions including cell migration, proliferation, apoptosis, transcriptional activation, angiogenesis, and Golgi organization and vesicle trafficking. In the present study, the molecular basis of Protein Kinase D recruitment to the Golgi complex was investigated. I had discovered that class I and Class II ARF proteins and more specifically ARF1 can function as a tethering factor for Protein Kinase D at the Golgi complex. These conclusions were based on protein-protein interaction assays, colocalization experiments, and Golgi functional assays after knockdown down of Protein Kinase D in cells. In this experiment, a His-tagged C1b domain of PKD2 was expressed and purified from bacteria. This recombinant protein was tested positive for His tag by Western Blotting with the His antibody from EMD Millipore. I had purchased this antibody because of my prior positive experiences with antibodies and other products from EMD Millipore.