Rockland Immunochemicals
anti-GFP FITC conjugated
600-402-215
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I used this antibody against GFP protein to enhance the GFP fluorescence in in mouse splenocytes as the population being detected is very rare.
Flow Cytometry
Mouse splenocytes
1:100 for 3 hours on ice
FBS/BSA/Fc block
NA
FITC channel on flowcytometer
FITC positive cells were detected that were usually difficult to detect. Use of isotype confirmed the presence of FITC positive cells in the population.
None
Signal is enhanced
Non specific binding can result if blocking is not proper
Overall good choice to replace direct GFP detection that tends to fade very easily.