CST HP1-Alpha Antibody Review

Genetics
Yale
Tech

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

HP1α Antibody

Catalog Number:

2616S

Our lab generates a lot of protein knockouts in mouse embryonic stem cells using crispr-cas9 technology. We use Western Blots and subsequent sequencing to screen potential knockout clones.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse ES cells

Primary Incubation

1:1000 4C overnight

Blocking Agent

5% BSA

Secondary Incubation

1:3000 in 5% BSA for one hour at RT

Tertiary Incubation

N/A

Detection

Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad

Results Summary

Great antibody, strong clear signal

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Clear strong band

The Bad

N/A

The Bottom Line

Great antibody, would use again.

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