Bio-Rad (Formerly AbD Serotec)
Rat Anti-Mouse CD3
MCA500GA
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Browse Similar Products: CD3 Immunohistochemistry Antibodies
Since the antibody is new, we have decided to try it on some frozen arteries and MS brain samples. So far it works well, but we are experimenting with the minimum dilution as well as quality of the tissue to determine the most efficient method.
Immunohistochemistry
Frozen Mouse Brain
1:100 overnight at 4 degrees celsius
Normal horse serum 2.5%
Pan specific anti mouse/rabbit/goat
Streptavidin complex
Nova Red
The results were decent, and we are off to a good start. Next we will experiment with the maximum dilution to use as well as different types of tissue samples.
None
Visually easy to detect positive areas
The quantity.
This is definitely a good product that we will use in the future, and continue to experiment with for maximum efficiency.