Roche Diagnostics, Germany
RNase A
10109169001
The aim of the study was to purify DNA from rat liver, amplify short reason of DNA using PCR and to clone in a suitable bacterial expression vector. For this, after DNA isolation it was subjected to RNase A treatment to remove any RNA contamination. RNase A was obtained from Roche Diagnostic because of its easy availability, target specificity and cost effective. After RNase A treatment short region of DNA was amplified and cloned into suitable vector.
Preparation of purified DNA and Cloning
Rat liver, phenol:chloroform: isoamyl alcohol, ethanol, isopropanol, DNA, nuclease free water.
DNA from rat liver was isolated using phenol:chloroform: isoamyl alcohol based standard protocol. To remove any RNA contamination 5 micL of RNase A (10 micg/mL) was added to 500 micL solution and incubated at 37 0C for 45 min. Purity of DNA was checked with the help of nonodrop. The short region of RNA free DNA was then amplified and the PCR product was cloned into vector.
None
The DNA was RNA free with purity (ʎmax 260/280) of nearly 1.9.
Helps to get RNA free DNA
No
Good and value for money RNase A from Roche Diagnostics