Novus
SUMO1 antibody
NBP2-32901
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This antibody was used in investigation of SUMOylation of the cystic fibrosis transmembrane conductance regulator (CFTR). Several antibodies were tested in this study including laboratory generated one and this product reviewed here (Novus, NBP2-32901) to enable detection of CFTR conjugated SUMO1.
Western Blot
IB3-1 cell transfected with CFTR
1:1000 dilution overnight at 4 degree in blocking reagent
3% BSA (fraction V, Sigma) in PBS
1:5000 dilution of anti-mouse HRP for 1hr at room temperature in blocking reagent
NA
Clarity Western ECL (BioRad), film
Antibody works OK in western blot, which shows CFTR conjugated SUMO1.
None
Detection of SUMO1
Background
Useful antibody for detection of CFTR conjugated SUMO1