Cayman chemical, USA
TBARS Assay Kit
10009055
The experiment was conducted to measure TBARS levels in plasma as a measure of lipid peroxidation. Plasma was separated from RBCs by centrifuging the blood. TBARS were estimated in plasma according to the manufacturer’s protocol.
TBARS detection in plasma
Plasma
Blood was collected in EDTA coated vaccutainers. Blood was centrifuged at 700 xg for 10 minutes to separate plasma from RBCs. TBARS level was measured in plasma according to manufacturer’s instructions. 100 µl of samples or standards were added to 100 µl of SDS solution in tubes and mixed well. 4 ml color reagent was added and tubes were boiled for 1 hour. Tubes were then transferred to ice to stop the reaction. After 10 minutes incubation, tubes were centrifuged at 1600 xg. The absorbance of clear supernatant was measured at 540 nm and the level of TBARS was calculated from the MDA standard. MDA standard (0, 0.625, 1.25, 2.5, 5, 10, 25, 50 µM) was prepared according to the kit instructions
Samples and standards should be kept at 4 °C
Results were highly reproducible
No
Very simple to perform
Simple and rapid estimation of lipid peroxidation by TBARS assay kit