IL-17 Detection Using Cytometric Bead Array

University of California
Internal Medicine
Assistant Project Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

IL-17 flex set CBA kit (Bead D6)

Catalog Number:

562174

We aimed at determining circulating IL-17 protein levels without having to do intracellular flow cytometry but still using the technique as well. BD CBA kit offers a good option to solve this as protein levels to femtograms are also possible to determine with CBA kits.

Experimental Design and Results Summary

Application

Bead based protein detection from serum/Multiplex using CBA

Starting Material

Serum samples from various mice injected with control and IL-23

Protocol Overview

Was provided along with the kit.

Tips

Mix beads thoroughly otherwise nothing will be detected.

Results Summary

Overall good and could detect very low quantities as well.

Additional Notes

None

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Summary

The Good

Fast detection.

The Bad

Requires an expensive software FCAP Array.

The Bottom Line

Expensive but fast way of multiplexing to determine the serum cytokines in both mice and humans.

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