Sigma Aldrich, USA
Xanthine oxidase activity assay kit
MAK078-1KT
The aim of the experiment was to measure the activity of xanthine oxidase in rat liver samples. The liver samples were homogenized and the activity of xanthine oxidase was measured in supernatant by following the kit protocol.
Kit is suitable for measuring xanthine oxidase activity in blood and in different tissues to assess the formation of uric acid.
Rat liver sample
Rat liver was homogenized in 4 volumes of xanthine oxidase assay buffer and centrifuged to remove insoluble material. The supernatant was used for the assay. The volume of the sample used was standardized to 20 µl and added to the wells of 96 microtitre plate. The volume was made upto 50 µl by adding xanthine oxidase assay buffer. The assay was performed according to the kit instructions. Hydrogen peroxide standard (2, 4, 6, 8 and 10 nmole) was prepared by following manufacturer’s instructions. One unit of xanthine oxidase is defined as the amount of enzyme that catalyzes the oxidation of xanthine, yielding 1.0 μmole of uric acid and hydrogen peroxide per minute at 25 °C.
Kit should be stored at -20°C
Results were obtained with high precision
No
Very simple to perform
Rapid detection of xanthine oxidase by Xanthine oxidase activity assay kit