Beta-Actin Antibody Santa Cruz Review

Genetics
Yale
Tech

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

β-Actin (C4)

Catalog Number:

sc47778

Our lab generates a lot of protein knockouts in mouse embryonic stem cells using crispr-cas9 technology. We use Western Blots with beta actin as loading control to screen knockouts.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse ES cells

Primary Incubation

1:1000 4C overnight

Blocking Agent

5% milk

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad

Results Summary

Great antibody, clear band. Don't need to add secondary. Easy to use. Can be diluted to 1:3000 and still give good results.

Additional Notes

None

Related Categories

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Summary

The Good

Clear band, strong antibody

The Bad

N/A

The Bottom Line

Great antibody, used in most of our WBs.

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