MLLT3 Rabbit Monoclonal Antibody Abnova Review

June 03, 2016

Genetics
Yale
Tech

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Abnova

Product Name:

MLLT3 rabbit monoclonal antibody

Catalog Number:

H00004300-K

 

Browse Similar Products: MLLT3 Western Blot Antibodies

Our lab generates a lot of protein knockouts in mouse embryonic stem cells using crispr-cas9 technology. We use Western Blots and subsequent sequencing to screen potential knockout clones.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse ES cells

Primary Incubation

1:1000 4C overnight

Blocking Agent

5% milk

Secondary Incubation

1:3000 in 5% milk for one hour at RT

Tertiary Incubation

N/A

Detection

Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad

Results Summary

Pretty good antibody with strong clear band that is strong in wildtype sample and weak in knockout samples. Faint nonspecific band close to specific band can complicate analysis if gel isn't run long enough.

Additional Notes

None

Image Gallery

Summary

The Good

Strong specific band

The Bad

Faint nonspecific band below specific band.

The Bottom Line

Good antibody for screening knockouts after your optimize conditions.

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