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FITC anti-human CD235a (Glycophorin A) Antibody
349104
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Cells yielded by enzymatic digestion synovial tissue were stained for CD235a to excluded erythrocytes from downstream analysis
Flow Cytometry
Human synovial cells
30 minute incubation at 4oC. Live/dead stain.
No specific blocking step. 2% FCS in staining buffer.
30 minute incubation at 4oC. Antibody cocktail used at this point containing antibody at 5ug/ml.
None
Mo Flow Astrios EQ 488 excitation 540/40 bandpass filter for emission
Antibody worked well enough to identify a clear positive population. The intensity of the staining was not that bright but was still clear enough to define a CD235a positive population
Antibody worked well an produced clear positive population
Staining is not very bright
Antibody allows identification of the CD235a positive cells even if the staining is not the brightest