Cell Signaling Technology
Caspase-1 Antibody
2225
Info: View Product View Product Specs
Browse Similar Products: Caspase-1 Western Blot Antibodies
Mammalian smooth cells grown in complete medium were growth arrested and treated with specific growth factor for different time periods. Cell extracts were prepared, resolved in SDS-PAGE, western transferred and immuno-detected for caspase-1 (alpha: 50kDa, beta: 20kDa, gamma: 20kDa, delta: 35kDa) levels using its specific antibodies obtained from Cell Signaling Technology. Selection of antibody was based on western blot picture information provided in antibody flyer.
Western Blot
Mammalian whole cell lysate
Dilution (1:1000) in 5% skimmed milk prepared in TBST, overnight incubation at 4 degree Celsius
5% skimmed milk prepared in TBST
Dilution (1:4000) in 5% skimmed milk prepared in TBST, 1hr at RT
NA
Enhanced chemiluminiscent detection in X-Ray film
Cell Signaling Technology antibody has not detected caspase-1 (alpha, beta, gamma, delta) at correct band size or band intensity.
None
Faint bands were observed
Failed to detect specific bands
Poor antibody for Immunoblotting in mammalian cells