Abcam
Anti-MST1/MST2 (phospho T183) antibody
ab79199
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Mouse pups were exposed to low oxygen for different time periods and tested for its effect on MST1/MST2 (phospho T183) phosphorylation. Retinal extract from treated and non treated mice pups were resolved in 10 % SDS-PAGE, and immunoblotted with Anti-MST1/MST2 (phospho T183) antibody purchased from Abcam. Selection of antibody was based MST1/MST2 (phospho T183) band shown in antibody flyer.Mouse pups were exposed to low oxygen for different time periods and tested for its effect on MST1/MST2 (phospho T183) phosphorylation. Retinal extract from treated and non treated mice pups were resolved in 10 % SDS-PAGE, and immunoblotted with Anti-MST1/MST2 (phospho T183) antibody purchased from Abcam. Selection of antibody was based MST1/MST2 (phospho T183) band shown in antibody flyer.
Western Blot
Mouse retinal whole tissue extract
Dilution (1:1000) in 5% BSA prepared in TBST, overnight incubation at 4 degree Celsius
5% skimmed milk prepared in TBST
Dilution (1:4000) in 5% skimmed milk prepared in TBST, 2hr at RT
None
ECL detection in X-Ray film
The antibody has detected very weak band of MST1/MST2 (phospho T183) protein at nearly 56 kDa molecular weight. An intense bright band was detected at molecular weight less than 29 kDa. However, no or little effect of treatment was observed in MST1/MST2 phosphorylation.
MST1/MST2 (phospho T183) protein was detected
Lot of non-specific bands were detected
Poor specificity of antibody