Satisfactory to Detect Total aB-Crystallin in Mouse Retina Samples

May 25, 2016

School of Pharmacy
University of Colorado, Denver
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Company:

Santa Cruz Biotechnology, Inc.

Product Name:

AlphaB-crystallin (A-4) Antibody

Catalog Number:

sc-365088

This antibody was found to be good to detect the total aB-Crystallin in mouse retina.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse retina

Primary Incubation

1:1000 overnight at 4oC

Blocking Agent

5% Skimmed milk powder in PBS

Secondary Incubation

1:500 2 hours at RT

Tertiary Incubation

NA

Detection

IR Dye

Results Summary

Got very distinct bands for this protein at expected molecular weight region. However, there were some non specific bands.

Additional Notes

None

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Summary

The Good

Less non-specific signals

The Bad

Needs to be used at high concentration.

The Bottom Line

I would recommend this antibody for detection of aB-Crystallin in retina samples by western blotting.

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