Great Separation of B Cells

Immunology
The Jackson Laboratory for Genomic Medicine
Lab Manager, RA III

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cy7 anti-human CD19 Antibody

Catalog Number:

302216

This antibody was used within a phenotyping panel to screen patients' blood. This antibody offers a perfect separation of CD3- CD19+ HLA-DR+ B cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

2/50 dilution in final volume of 50ul (PBS+ 2%FBS) for 15min at RT at dark.

Blocking Agent

FcR block (Miltenyi) for 3min at RT

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

LSRII (Becton Dickinson)

Results Summary

Patient's blood samples were analyzed with flow jo, and B cells were easily isolated across samples.

Additional Notes

Stock concentration of 100ug/ml was diluted to a working concentration of 20ug/ml for a final concentration of 1ug/ml during staining.

Related Categories

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Summary

The Good

Nice separation

The Bad

Too concentrated, needs to dilute

The Bottom Line

Great antibody for B cells separation.

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