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Foxp3 staining buffer kit
00-5523
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Intracellular Foxp3 were stained to evaluate T regulatory cell (Treg) population in dogs with cancer before and after treatment . We found a significantly higher percentage of Treg in dogs with cancer compared with healthy dogs. Although treatment was unable to decrease percentage of Tregs as in healthy dogs, percentage of Tregs was reduced in the dogs with cancer.
Clinical Cancer Research
Blood
After staining with CD4 and CD25, cell were resuspended in 100ul of “cytofix/cytoperm” solution and incubated on ice for 2hrs in the dark. After washing at the end of the incubation time, cells were resuspended and incubated with fluorescent conjugated anti-Foxp3.
Incubate on ice for 2hrs in dark with "cytofix/cytoperm” solution.
We found a significantly higher percentage of Treg in dogs with cancer compared with healthy dogs. Although our treatment was unable to decrease the percentage of Tregs as in healthy dogs, percentage of Treg was reduced in the dogs with cancer.
None
Time saving, easy to perform.
Easy to use, saves time compared with other traditional agents. Also work for other assays for intracellular staining.
Product Name: Foxp3 / Transcription Factor Staining Buffer Set KitSupplier Page