Complex Software for this Flow Cytometer

February 16, 2016

BlueskyReddit
University of Rochester
Immunology
Graduate Student

Overall

Performance

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD LSRFortessa

Catalog Number:

None

Using the Facsdiva software is very complex--a lot you have to learn and get used to. Once all the parameters and tubes are set up on the software, it is pretty easy to run your actual samples.

Experimental Design and Results Summary

Tips

Set up single stain controls prior to experimental sample tubes so that the brightest peak is not out of the axis.

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Summary

The Good

Convenient and can be quick

The Bad

A lot of settings (like compensating post acquisition) that need to be more intuitive to get to

The Bottom Line

Good machine for acquisition. Cleanup procedure is required after every person. Better than Calibur because Fortessa has more parameters. For analysis, turn to another software.

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