Complex Software for this Flow Cytometer

February 16, 2016

University of Rochester
Immunology
Graduate Student

Overall

Performance

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD LSRFortessa

Catalog Number:

None

Using the Facsdiva software is very complex--a lot you have to learn and get used to. Once all the parameters and tubes are set up on the software, it is pretty easy to run your actual samples.

Experimental Design and Results Summary

Application

Flow acquisition

Starting Material

Primary cells, hybridoma cells

Protocol Overview

HEK293 cells were incubated with three different dilutions of a Thy1.1-expressing lentivirus. Cells were stained for Thy1.1 and acquired on the Fortessa.

Tips

Set up single stain controls prior to experimental sample tubes so that the brightest peak is not out of the axis.

Results Summary

This is a good machine for single or multi-color flow. The machine could be more user friendly and more intuitive. Adjusting compensation can be complicated or impossible at a few occasions. Analysis on this is poor compared to Flowjo. Gets clogged frequently.

Features Summary

Has both facs tube and plate setting

Additional Notes

None

Image Gallery

Summary

The Good

Convenient and can be quick

The Bad

A lot of settings (like compensating post acquisition) that need to be more intuitive to get to

The Bottom Line

Good machine for acquisition. Cleanup procedure is required after every person. Better than Calibur because Fortessa has more parameters. For analysis, turn to another software.

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