Anti-ß-Actin Antibody from Sigma-Aldrich

February 18, 2016

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

Anti-β-Actin antibody

Catalog Number:

A1978

Browse Similar Products: Anti-beta-Actin Western Blot Antibodies

This antibody was used to detect expression of β-Actin as a loading control for western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Cancer Cells whole lysates

Primary Incubation

1:2000, 2 hours, RT

Blocking Agent

Non-fat milk

Secondary Incubation

1:5000, 1 hour, RT

Tertiary Incubation

NA

Detection

Chemiluminescence detection kit

Results Summary

This antibody can be used to detect the expression of β-Actin as a loading control for Western Blot.

Additional Notes

None

Related Categories

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Summary

The Good

Signal is strong and specific.

The Bad

None

The Bottom Line

This is a perfect loading control antibody for Western Blot.

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