PE/Cy7 Anti-Human CD5 Antibody, BioLegend, Clone UCHT2

Genetics and Genomic Sciences
Icahn School of Medicine at Mount Sinai
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cy7 anti-human CD5 Antibody

Catalog Number:

300621

I am evaluating the expression of CD4, CD5 and CD7 on Jurkat cells after CRISPR/Cas9 mediated knock-out as a proof-of-principle experiment. We were trying to establish whether the knockout works, and it was easiest to address that by looking at the surface receptors. This product gave very nice staining of human Jurkat cell line.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Jurkat cells

Primary Incubation

1:100, 30 min on ice

Blocking Agent

0.5% BSA, 4% FBS

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Fluorescence

Results Summary

Good staining of CD5 expression on Jurkat cells

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

None

The Bottom Line

Simple, quick, specific

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