Cell Signaling Technology
Acetyl-STAT3 (Lys685) Antibody
2523
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Mammalian cell lines were treated with growth factor in a time dependent manner, and tested for STAT-3 Lys685 acetylation using western blotting. Cell Signaling antibody was selected because it can detect steady state levels of STAT-3 acetylation in non-transfected cells.
Western Blot
Mammalian whole cell lysate
Dilution (1:1000) in 5% BSA prepared in TBST, overnight incubation at 4 degrees Celsius
5% skimmed milk prepared in TBST
Dilution (1:5000) in 5% skimmed milk prepared in TBST, 1hr at RT
None
Enhanced chemiluminiscence detection in X-Ray film
In the present study, antibody from cell Signaling technology was used for immunodetection and showed excellent specificity towards Ac-STAT3 in mammalian cells. However, no response of growth factor treatment on STAT-3 (Lys685) acetylation was observed.
Detected highly specific band of Ac-STAT3
Excellent antibody for detection