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Pacific Blue™ anti-human HLA-DR Antibody
307624
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The aim of this study was a phenotypic analysis of human peripheral blood using multicolor flow cytometry.
Flow Cytometry
Human peripheral blood
50ul of blood was incubated for 30 minutes at room temperature in the dark with the antibody. After this peripheral blood was lysed using the BD lysing buffer as indicated in the manual. Cells were washed three times with PBS and then resuspended in 300ul of FACS buffer and used for flow cytometry.
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Flow cytometry
HLA-DR positive cells were clearly identified with this antibody. In the figure you see HLA-DR expression on monocytes.
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Good staining of HLA-DR positive cells
This antibody serves as a good tool when performing phenotypic analysis of immune cells using flow cytometry.