BD Biosciences
V500 Mouse Anti-Human CD4
560768
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The aim of this study was a multicolor immunophenotypic analysis of peripheral blood of children. This antibody was selected because of its conjugate (V500).
Flow Cytometry
Human peripheral blood
50ul of blood was incubated for 30 minutes at room temperature in the dark with the antibody. After this peripheral blood was lysed using the BD lysing buffer as indicated in the manual. Cells were washed three times with PBS and the resuspended in 300ul of FACS buffer and then used for flow cytometry
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Flow cytometry
This antibody provides a good staining of CD4+ T cells in human peripheral blood is an excellent tool for multicolor flow cytometry
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Clear identification of CD4+ T cells
This antibody identifies CD4+ T cells in human peripheral blood very well.