Sigma-Aldrich
Monoclonal Anti-Tubulin, acetylated antibody produced in mouse
T6793
Browse Similar Products: Acetylated Tubulin Immunofluorescence Antibodies
Primary cilia are microtubule based structures that range from 1-15 microns and are present in almost every cell in our body. They have signaling hubs for various pathways and most importantly the sonic hedgehog pathway. I am interested in understanding the mechanism of hedgehog signal transduction. I routinely perform immunofluorescence staining to study trafficking of hedgehog signaling components in response to pathway stimulation. To visualize primary cilia, acetylated tubulin antibody is widely used and I had selected this marker based on previous publications.
Immunofluorescence
NIH3T3 cells
1:3000 dilution in blocking agent for 1 hr at room temp.
Blocking buffer: 1% donkey serum + 1% BSA + 0.1% Triton X-100, in PBS.
Donkey anti-mouse Alexa Fluor 647 at 1:1000 dilution in blocking agent for 1 hr at room temp.
NA
Images were acquired on a Leica TCS SP8 confocal microscope with a 63X oil immersion objective.
Confluent NIH3T3 cells were serum starved for 24 hours to induce primary cilia. Cells were fixed in 4% PFA. This antibody nicely stains primary cilia.
None
A nice marker for visualization of primary cilia.
Very good marker for primary cilia